Books

Dunny, G. and S. C. Winans (eds). 1999. Cell-Cell Signaling in Bacteria. ASM Press, Washington, D. C.

Host Recognition within Crown Gall Tumors

Jafri, S., Evoy, S., Cho, K., Craighead, H. G., and Winans, S. C. 1999. A proline-responsive member of the Lrp family of transcriptional regulators condenses 100 nucleotides of DNA into a globular nucleoprotein complex. J. Mol. Biol. 288:811-824.

Lyi, S. M., S. Jafri, and S. C. Winans. 1999. Mannopinic acid and agropinic acid catabolism region of the octopine-type Ti plasmid pTi15955. Mol. Microbiol. 31:339-348.

Cho, K., C. Fuqua, and S. C. Winans. 1997. Transcriptional regulation and location of Agrobacterium tumefaciens genes required for complete catabolism of octopine. J. Bacteriol. 179:1-8.

Cho, K., and S. C. Winans. 1996. The putA gene of Agrobacterium tumefaciens is transcriptionally activated in response to proline by an Lrp-like protein and is not autoregulated. Mol. Microbiol. 22:1025-1034.

Cho, K., W.C. Fuqua, B.S. Martin, and S.C. Winans. 1996. Identification of Agrobacterium tumefaciens genes that direct the complete catabolism of octopine. J. Bacteriol. 178:1872-1880.

Wang, L. and S.C. Winans. 1995. The sixty nucleotide OccR operator contains a subsite essential and sufficient for OccR binding and a second subsite required for ligand-responsive DNA bending. J. Mol. Biol. 253:691-702.

Wang, L. and S.C. Winans. 1995. High angle and ligand-induced low angle DNA bends incited by OccR lie in the same plane with OccR bound to the interior angle. J. Mol. Biol. 253:32-38.

Cho, K. and S.C. Winans. 1993. Altered function mutations in the Agrobacterium tumefaciens OccR protein and in an OccR-regulated promoter. J. Bacteriol. 175:7715-7719.

Wang, L., J.D. Helmann, and S.C. Winans. 1992. The A. tumefaciens transcriptional activator OccR causes a bend at a target promoter, which is partially relaxed by a plant tumor metabolite. Cell 69:659-667.

Valdivia, R.H., L. Wang, and S.C. Winans. 1991. Characterization of a putative periplasmic transport system for octopine accumulation encoded by Agrobacterium tumefaciens Ti plasmid pTiA6. J. Bacteriol. 173:6398-6405.

Habeeb, L., L. Wang, S.C. Winans. 1991. Transcription of the octopine catabolism operon of the Agrobacterium tumor-inducing plasmid pTiA6 is activated by a LysR-type regulatory protein. Molec. Plant-Microbe Interact. 4:379-385.

Quorum Sensing

Zhu, J. and S. C. Winans. 1999. Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases traR turnover rates in whole cells. Proc. Natl. Acad. Sci U.S.A. 96: 4832-4837.

Zhu, J., J. W. Beaber, M. I. Moré, C. Fuqua, A. Eberhard, and S. C. Winans. 1998. Analogs of the autoinducer 3-oxo-octanoyl-homoserine lactone strongly inhibit activity of the TraR protein of Agrobacterium tumefaciens. J. Bacteriol. 180:5398-5405.

Zhu, J., and S. C. Winans. 1998. Activity of the quorum-sensing regulator TraR of Agrobacterium tumefaciens is inhibited by a truncated, dominant defective TraR-like protein. Mol. Microbiol. 27:289-297.

More, M. I., L. D. Finger, J. L. Stryker, C. Fuqua, A. Eberhard, S. C. Winans. 1996. Enzymatic synthesis of a quorum-sensing autoinducer using defined substrates. Science 272:1655-1658.

Fuqua, W.C. and S.C. Winans. 1996. Localization of OccR-activated and TraR-activated promoters that express two ABC-type permeases and the traR gene of Ti plasmid pTiR10. Mol. Micro. 20:1199-1210.

Fuqua, W.C. and S.C. Winans. 1996. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J. Bacteriol. 178:435-440.

Alt-Morbe, J., J. L. Stryker, C. Fuqua, S. K. Farrand, and S. C. Winans. 1996. The conjugal transfer system of A. tumefaciens octopine-type Ti plasmids is closely related to the transfer system of an IncP plasmid and distantly related to Ti plasmid vir genes. J. Bacteriol. 178:4248-4257.

Fuqua, W.C., M. Burbea, and S.C. Winans. 1995. Activity of the Agrobacterium Ti plasmid conjugal transfer regulator TraR is inhibited by the product of the traM gene. J. Bacteriol. 177:1367-1373.

Fuqua, W.C. and S.C. Winans. 1994. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J. Bacteriol. 176:2796-2806.

Host Recognition at Wound Sites

Kalogeraki, V. S. J. Zhu, A. Eberhard, E. L. Madsen, and S. C. Winans. 1999. The phenolic vir pene inducer ferulic acid is O-demethylated by the VirH2 protein of an A. tumefaciens Ti plasmid. Mol. Microbiol. (in press).

Chang, C.-H., J. Zhu, and S. C. Winans. 1996. Pleiotropic phenotypes caused by genetic ablation of the receiver module of the Agrobacterium tumefaciens VirA protein. J. Bacteriol. 178:4710-4716.

Chang, C.-H. and S. C. Winans. 1996. Resection and mutagenesis of the acid pH-inducible P2 promoter of the Agrobacterium tumefaciens virG gene. J. Bacteriol. 178:4717-4720.

Kalogeraki, V.S. and S.C. Winans. 1995. The octopine-type Ti plasmid pTiA6 of Agrobacterium tumefaciens contains a gene homologous to the chromosomal virulence gene acvB. J. Bacteriol. 177:892-897.

Han, D.C. and S.C. Winans. 1994. A mutation in the receiver domain of the Agrobacterium tumefaciens transcriptional regulator VirG increases its affinity for operator DNA. Mol. Microbiol. 12:23-30.

Mantis, N.J. and S.C. Winans. 1993. The chromosomal regulatory gene chvI of Agrobacterium tumefaciens complements an Escherichia coli phoB mutation and is required for virulence. J. Bacteriol. 175:6626-6636.

Chang, C.-H. and S.C. Winans. 1992. Functional roles assigned to the periplasmic, linker, and receiver domains of the Agrobacterium tumefaciens VirA protein. J. Bacteriol. 174:7033-7039.

Han, D.C., C.-Y. Chen, Y.-F. Chen, and S.C. Winans. 1992. Altered function mutations of the transcriptional regulatory gene virG of Agrobacterium tumefaciens. J. Bacteriol. 174:7040-7043.

Mantis, N.J. and S.C. Winans. 1992. The Agrobacterium vir gene transcriptional activator virG is transcriptionally induced by acid pH and other stress stimuli. J. Bacteriol. 174:1189-1196.

Mantis, N.J. and S.C. Winans. 1992. Characterization of the Agrobacterium tumefaciens heat shock response: evidence for a 32-like sigma factor. J. Bacteriol. 174:991-997.

Chen, C.-Y., L. Wang, and S.C. Winans. 1991. Characterization of the supervirulent virG gene of the Agrobacterium tumefaciens plasmid pTiBo542. Mol. Gen. Genet. 230:302-309.

Chen, C-Y. and S.C. Winans. 1991. Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter. J. Bacteriol. 173:1139-1144.

Winans, S.C. 1990. Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolics, phosphate starvation, and acidic growth media. J. Bacteriol. 172:2433-2438.

Plasmid pKM101 of Enteric Bacteria

Paterson, S., M. I. Moré, G. Pillay, C. Cellini, R. Woodgate, G. C. Walker, V. N. Iyer, and S. C. Winans. 1999. Genetic analysis of the mobilization and leading regions of the IncN plasmids pKM101 and pCU1. J. Bacteriol. 181: 2572-2583.

Cellini, C., V. S. Kalogeraki, and S. C. Winans. 1997. The hydrophobic TraM protein of pKM101 is required for conjugal transfer and sensitivity to donor-specific bacteriophage. Plasmid 37:181-187.

More, M. I., R. F. Pohlman, and S. C. Winans. 1996. Genes encoding the pKM101 conjugal mating pore are negatively regulated by the plasmid-encoded KorA and KorB proteins. J. Bacteriol. 178:4392-4399.

Pohlman, R.F., H.D. Genetti, and S.C. Winans. 1994. Common ancestry between IncN conjugal transfer genes and macromolecular export systems of plant and animal pathogens. Mol. Micro. 14:655-668.

Pohlman, R.F., F. Liu, L. Wang, M.I. More and S.C. Winans. 1993. Genetic and biochemical analysis of an endonuclease encoded by the IncN plasmid pKM101. Nucleic Acids Res. 21:4867-4872.

Reviews

Winans, S. C. and J. Zhu. 2000. The roles of cell-cell communication in confronting the limitations and opportunities of high population densities. In Bacterial Stress. G. Storz and R. Hengge-Aronis (eds). ASM Press, Washington, D. C.

Winans, S. C., V. Kalogeraki, S. Jafri, R. Akakura, and Q. Xia. 1999. Diverse roles of Agrobacterium Ti plasmid-encoded genes in the formation and colonization of plant tumors. in Pathogenicity Islands. J. B. Kaper and J. Hacker (eds). AMS Press, Washington, D. C.

Winans, S. C., J. Zhu, and M. I. Moré. 1999. Cell density-dependent gene expression by Agrobacterium tumefaciens during colonization of crown gall tumors. in Cell-Cell Signaling in Bacteria. G. M. Dunny, and S. C. Winans (Eds). ASM Press, Washington, D. C.

Fuqua, C., S. C. Winans, E. P. Greenberg. 1996. Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators. Ann. Rev. Microbiol. 50:727-751.

Winans S. C., L. Wang, P. Dwen, C. Fuqua, M. I. Moré, J. Alt-Mörbe, J. Styker, and M. Burbea. 1996. Transcriptional regulation of conjugal transfer genes of octopine-type Ti plasmids. in Crown Gall: Advances in Understanding Interkingdom Gene Transfer. W. Ream and S.B. Gelvin (Eds). American Phytopathology Society, St Paul.

Winans, S. C., D. L. Burns, P. J. Christie. 1996. Adaptation of a conjugal transfer system for the export of pathogenic macromolecules by Agrobacterium tumefaciens and Bordetella pertussis. Trends Microbiol. 4:64-68.

Winans, S.C., N.J. Mantis, C.-Y. Chen, C.-H. Chang, and D.C. Han. 1994. Host recognition by the VirA, VirG two-component regulatory proteins of Agrobacterium tumefaciens. Research Microbiol. 145:461-473.

Fuqua, W.C., S.C. Winans, and E.P. Greenberg. 1994. Quorum sensing in bacteria: the LuxR/LuxI family of cell density responsive transcriptional regulators. J. Bacteriol. 176:269-275.

Winans, S.C. 1992. Two-way chemical signaling in Agrobacterium-plant interactions. Microbiol. Rev. 56:12-31.

Winans, S.C. 1991. An Agrobacterium two-component regulatory system for the detection of plant wounds. Molec. Microbiol. 5:2345-2350.